Improving Membrane Staining of Cultured Cells Using Ferrocyanide as a Post-Fixative
نویسندگان
چکیده
منابع مشابه
A Versatile Method for Immunofluorescent Staining of Cells Cultured on Permeable Membrane Inserts
BACKGROUND Obtaining high-quality images of cellular structures via immunofluorescence staining is critical for cellular localization studies. Often, these studies cannot be performed in parallel with certain oncology, virology, pharmacokinetic, and drug absorption studies due to model system technicalities requiring the cells to be cultured on porous membranes rather than glass or plastic. MAT...
متن کاملElectronic osmometer with rigid membrane of copper ferrocyanide.
greatly reduced the amount of the sample solution and the measuring time.1-3 However, the electronic osmometers reported to date are in use of flaccid mem branes which result in baseline instability and limit the use to low con centrations of colloid solute in solutions. Recently, we have designed an electronic osmometer with a rigid semiperme able membrane made from a baked clay disc permeated...
متن کاملRapid fixation and immunofluorescent staining of cultured cells using microwave irradiation
Microwave irradiation during tissue fixation and immunostaining reduces the sample preparation time. Microwave irradiation also facilitates the penetration of fixatives and antibody solutions into the tissues, resulting in efficient fixation and reduction of non-specific antibody binding, respectively. Experimental procedures involving immunofluorescence microscopy are time-consuming as this me...
متن کاملHistopathologic evaluation of mast cells in reticular and erosive oral lichen planus using toluidine blue staining
Introduction: Oral Lichen Planus (OLP) is one of the most common mucocutaneous lesion, and its etiologic factors have not been elucidated yet. OLP has two different clinical forms including reticular and erosive. Mast cells function in non-specific immunology is an etiologic factor involved in the formation of lichen planus. This study aimed at determining the pattern of mast cells presence in ...
متن کاملUsing WPT as a New Method Instead of FFT for Improving the Performance of OFDM Modulation
Orthogonal frequency division multiplexing (OFDM) is used in order to provide immunity against very hostile multipath channels in many modern communication systems.. The OFDM technique divides the total available frequency bandwidth into several narrow bands. In conventional OFDM, FFT algorithm is used to provide orthogonal subcarriers. Intersymbol interference (ISI) and intercarrier interferen...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Microscopy Today
سال: 2007
ISSN: 1551-9295,2150-3583
DOI: 10.1017/s1551929500051014